Development and Validation of the Quantification Method for Hydroxychloroquine in Volumetric Absorptive Microsampling (VAMS) Using High-Performance Liquid Chromatography-Photodiode Array

Table 2

Data of calibration curve of HCQ.

Concentration (ng/ml)	0.00	2.00	10.00	50.00	100.00	500.00	1000.00	3000.00	6500.00	Slope	Intercept	r
Replicate	Measured concentration (ng/ml)
1	0	2.14	9.45	51.76	111.46	561.40	913.73	3095.51	6464.28	0.00142	0.06763	0.9997
2	0	1.91	9.67	49.22	105.13	555.91	917.22	3154.84	6436.90	0.00138	0.09504	0.9995
3	0	1.99	10.27	48.62	96.28	535.52	947.38	3194.42	6415.70	0.14250	0.00162	0.9993
Mean (ng/mL)	0.00	2.01	9.80	49.87	104.29	550.94	926.11	3148.26	6438.96
SD	0.00	0.12	0.42	1.67	7.62	13.64	18.50	49.78	24.35
CV (%)	0.00	5.90	4.31	3.34	7.31	2.48	2.00	1.58	0.38