Evaluation of the Antimicrobial Effect of the Extracts of the Pods of Piliostigma thonningii (Schumach.) Milne-Redh. (Fabaceae)

Figure 1

A typical microbroth dilution assay showing MTT results after subculture of M. smegmatis with control drug rifampicin and hexane and ethanol pod extracts of P. thonningii. Viable M. smegmatis cells reduced yellow MTT dye to blue. This indicated that there was no inhibition of M. smegmatis in wells with blue colour. Column 11 represents cells only well (positive control). Column 12 containing media only remained yellow. Thus, no contamination of media occurred. The top two rows A and B remained yellow, where extract and media were checked for contamination.