Passiflora mollissima Seed Extract Induced Antiproliferative and Cytotoxic Effects on CAL 27 Spheroids

Figure 2

Spheroids of CAL 27 treated and untreated and their three-dimensional reconstruction. Box and Whiskers graphics show the most representative images of the spheroids’ morphological changes on parameters measurement before and after the PME treatment (10, 50, and 100 μg/ml) and 20 μM cisplatin 72 h. (a) Sphericity. (b) Solidity. (c) Diameter. (d) Volume. (e) Inverted microscopy images of spheroids and its tridimensional reconstruction with AnaSP software show density color schematically with voxel localization within the three-dimensional image. Scale bar: 200 μm.

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