Passiflora mollissima Seed Extract Induced Antiproliferative and Cytotoxic Effects on CAL 27 Spheroids

Figure 8

PME induces cell cycle arrest and regulates the expression of genes in CAL-27 spheroids. (a) P53 and HIF1-alpha mRNA expression in CAL 27 spheroids PME-treated (10, 50, and 100 μg/ml) compared to the untreated control and 20 μM cisplatin (b) MMP9 and CDH1 mRNA expression in CAL 27 spheroids. (c) The percentage of cells in G0/G1, G2/M, and S cell cycle phases analysis of CAL 27- spheroids following treatment for 72 h by flow cytometry. (d) Representative histogram of flow cytometry cell cycle. The results shown are representative of three independent experiments. p < 0.05 and p < 0.005, control versus PME-treated cells.

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