Advances in Pharmacological and Pharmaceutical Sciences

Research Article

Synergistic Antibacterial Effect of Ethyl Acetate Fraction of Vernonia amygdalina Delile Leaves with Tetracycline against Clinical Isolate Methicillin-Resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa

Phytochemical constituent result of EAFVA with LC-HRMS.


No.	Name	Formula	Molecular weight	Retention time (min)

1	3-Methoxy-5,7,3′,4′-tetrahydroxy-flavone	C₁₆H₁₂O₇	316.05678	0.834
2	Tectorigenin	C₁₆H₁₂O₆	300.06191	0.843
3	4-Methoxycinnamic acid	C₁₀H₁₀O₃	160.05173	5.216
4	5,7-Dihydroxy-2-(3-hydroxy-4[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-hydroxymethyl) oxan-2-yl]oxy}phenyl)4H-chromen-4-one	C₂₁H₂₀O₁₁	448.09871	7.192
5	Quercetin	C₁₅H₁₀O₇	302.04122	7.844
6	Apigetrin	C₂₁H₂₀O₁₀	432.10396	7.918
7	Baicalin	C₂₁H₁₈O₁₁	446.08296	7.999
8	Kuromanin	C₂₁H₂₀O₁₁	448.09871	8.282
9	3-Methoxy-5,7,3′,4′-tetrahydroxy-flavone	C₁₆H₁₂O₇	316.05678	8.786
10	Luteolin	C₁₅H₁₀O₆	286.04613	9.602
11	Diosmetin	C₁₆H₁₂O₆	300.06191	9.934
12	Apigenin	C₁₅H₁₀O₅	270.05138	10.764
13	Ingenol-3-angelate	C₂₅H₃₄O₆	430.23345	11.930
14	Chrysin	C₁₅H₁₀O₄	254.05678	13.296
15	Glycitein	C₁₆H₁₂O₅	284.06722	13.642