Development and Characterization of PEGylated Chromatographic Monoliths as a Novel Platform for the Separation of PEGylated RNase a Isomers

Figure 1

Breakthrough curves of PEGylated monoliths using BSA. The two ammonium sulfate concentrations used were 1.5 and 2.0 M and are represented by solid lines and dashed lines respectively. The color of the lines indicate the different BSA concentrations tested (blue lines, 1.0 mg/mL; red lines, 2.0 mg/mL and black lines, 3.0 mg/mL). Each column of graphs corresponds to each of the two Flow rates used (1.0 and 2.0 mL/min). All presented curves are the mean of three replicates.