Development and Characterization of PEGylated Chromatographic Monoliths as a Novel Platform for the Separation of PEGylated RNase a Isomers

Figure 2

Separation of PEGylated Ribonuclease A using PEGylated monoliths. Chromatogram at 215 nm of Ribonuclease A PEGylation reactions with different PEG molecular weights 5.0 (blue line), 20.0 (orange line) and 40.0 kDa (green line) were separated using PEGylated monoliths modified with different PEG molecular weights 1.0, 10.0, and 20.0 kDa. Each experiment was carried out by triplicate. Conductivity was also measured and is presened in the chromatograms (black dashed line). ^∗mono-PEGylated protein, ^∗∗di-PEGylated protein.