Development and Characterization of PEGylated Chromatographic Monoliths as a Novel Platform for the Separation of PEGylated RNase a Isomers

Table 1

Molar modification percentage of EDA disk monoliths with PEG through reductive amination and dynamic binding capacity of the PEGylated monoliths.

PEG size (kDa)	Monolith molar modification (%)			(mg BSA/mL resin)
		Flow rate (mL/min)		1			2
				Ammonium sulfate (M)
		BSA (mg/mL)		1.5	2	1.5		2
1	39.76	1		1.13 ± 0.05	2.67 ± 0.03	1.72 ± 0.05		3.21 ± 0.03
		2		1.68 ± 0.03	4.12 ± 0.14	2.71 ± 0.18		5.08 ± 0.05
		3		2.75 ± 0.02	5.09 ± 0.15	3.51 ± 0.18		6.85 ± 0.21
10	62.18	1		0.80 ± 0.02	2.72 ± 0.01	1.19 ± 0.04		2.92 ± 0.03
		2		1.33 ± 0.06	3.89 ± 0.04	1.92 ± 0.08		4.52 ± 0.04
		3		1.56 ± 0.03	5.07 ± 0.05	2.54 ± 0.07		5.75 ± 0.03
20	52.24	1		0.93 ± 0.01	3.18 ± 0.02	1.10 ± 0.02		3.02 ± 0.13
		2		1.90 ± 0.01	5.16 ± 0.01	2.20 ± 0.01		5.27 ± 0.03
		3		2.89 ± 0.05	6.26 ± 0.02	3.08 ± 0.19		6.88 ± 0.09

Data of presented represents the mean of three runs and their standard error.