Purification, Preliminary Structural Characterization, and In Vitro Inhibitory Effect on Digestive Enzymes by β-Glucan from Qingke (Tibetan Hulless Barley)

Table 1

The enzyme assay parameters.

Enzyme assay	Sample	Substrate		Enzyme		Buffer (pH)
	Vol. (μl)	Final Conc.	Vol. (μl)	Final Conc.	Vol. (μl)
α-Amylase	200	1.5%	400	0.1 μg/ml	200	Tris-HCl (pH 7.0)
Invertase	200	50 mM	200	1 μg/ml	200	Tris-HCl (pH 4.5)
α-Glucosidase	20	1.25 mM	20	0.01 unit	40	PBS (pH 6.8)
Lipase	300	0.15 mg/ml	600	1 mg/ml	300	Tris-HCl (pH 8.0)