3D In Vitro Human Organ Mimicry Devices for Drug Discovery, Development, and Assessment

Figure 2

Formation of the biomimetic liver sinusoid on a chip. (a) Schematic diagram of a liver sinusoid. Hepatic plates and endothelial cell (EC) monolayers are the main part of this unit. (b) Injection of cell-laden collagen and medium with factors. (c) The 3D schematic diagram of collagen and medium injection process. (d) After the stimulation of factors for two days, an EC monolayer formed and medium with factors was changed to medium without factors, forming the liver sinusoid on a chip. Reproduced (or adapted) with permission [41], ©2018, IOP Science.