3D In Vitro Human Organ Mimicry Devices for Drug Discovery, Development, and Assessment

Figure 5

Design principles of a tissue incubator. Multilayered PLCs were deposited on PDMS membrane through a stencil (a) to create a biological growing template (b) and a hexagonal contour (c). After overnight culture, the stencil was removed, and a hydrophilic diverter was assembled with a reservoir to create micro-/macrocirculation (d). The hydrophilic diverter provides PLCs with vertical anchorage. (e) Photograph of the entire device. (f) Schematic diagram of the radial flow. (g) Liver-on-a-chip (LOC) shows the cell viability (green). Reproduced (or adapted) with permission [42], ©2017, Wiley Online Library.