Nuclear-Targeting Delivery of CRISPRa System for Upregulation of β-Defensin against Virus Infection by Dexamethasone and Phenylalanine Dual-Modified Dendrimer

Figure 8

Ex vivo fluorescence immunohistochemical analyses (a) and histological characteristics (H&E stainning) (b) of lung sections from mice receiving different formulations (48 h after the inhalation). In the immunohistochemical assay, the nucleus, dCas9 protein, and β-defensin protein were stained blue, green, and red fluorescence, respectively. Inhalation dose: 5 μg/μL dCas9 plasmid and 5 μg/μL sgRNA (sgMBD4) in 100 μL PBS. Complexes were prepared at N/P value of 8. Scale bars, 100 μm in (a) and 50 μm in (b).

(a)

(b)