Supramolecular Biopolymers for Tissue Engineering

Figure 5

(a) It is possible to generate ultralong DNA chains using circular DNA templates. The strategy of elongation employed is a double rolling circle amplification (double RCA). Using two partially complementary DNA templates, the ultralong chains are able to hybridize with each other after mixing. (b) The use of stained DNA chains allows the observation of the hybridization process after being mixed. (c) The template DNA also contained aptamers that bind to a specific surface membrane protein (ALPL) of marrow cells. The authors designed a mechanism of capture and release of stem cells using the aptamer-containing ultralong DNA hydrogel and DNases for degradation of the matrix. These figures have been reproduced with permission from the American Chemical Society [114].

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