Establishing a Markerless Genetic Exchange System for Methanosarcina mazei Strain Gö1 for Constructing Chromosomal Mutants of Small RNA Genes

Figure 5

Analysis of protein expression pattern of ΔsRNA₁₅₄ versus wild type (a) under nitrogen limitation and (b) under nitrogen sufficiency. The M. mazei strains ΔsRNA₁₅₄, Δhpt, and wild type were grown in 50 mL medium under standard nitrogen-limiting conditions or under nitrogen sufficiency (see Figure 4). Cells were harvested in exponentially phase. Equal amounts of cell extracts (65 𝜇g) were applied to a 12% SDS-PA gel, which was subsequently stained with Coomassie blue. Arrows 1–3 indicate protein bands with differential expression in M. mazei ΔsRNA₁₅₄ and wild-type strains. Marker: LMW marker GE healthcare.

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(b)