Improving the Catalytic Activity of Hyperthermophilic Pyrococcus horikoshii Prolidase for Detoxification of Organophosphorus Nerve Agents over a Broad Range of Temperatures
Figure 5
Pot-life activity of WT-Ph1prol and prolidase mutants incubated anaerobically at 70°C. Pot-life experiments were performed in duplicate and samples were taken at 0, 1, 7, 14, 16, and 21 days to assess reactivity. Prolidase assays were performed in triplicate at 100°C and contained 0.2 mM CoCl2 and 4 mM Met-Pro. Error bars in Figure 5 represent standard deviation of specific activity across duplicate runs.
