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Research Article

Molecular Characterization of Copper and Cadmium Resistance Determinants in the Biomining Thermoacidophilic Archaeon Sulfolobus metallicus

Table 2

Proteins upregulated in S. metallicus cells exposed to 100 mM Cu or 1 mM Cd.

SpotMolecular weight (kDa)Cu induction levelsPutative function Microorganism relatedAccesion number

126.6Proteasome subunit S. solfataricus AAK41034
370.1Ferredoxin oxidoreductase S. solfataricus AAK42926
442.9Alcohol dehydrogenase S. solfataricus AAK43154
5352.6Hypothetical proteinS. acidocaldarius YP_254976
627Hypothetical protein P. aerophilum NP_559889
957Phosphoglycerate dehydrogenase M. thermautotrophicus AAB85466
13273.4Unknown
1428Unknown
15355.5Unknown
2046.1Glutamate dehydrogenase S. solfataricus AAK42230
2145Hypothetical protein M. acetivorans NP_618380.1
2249Hypothetical proteinP. torridus YP_022807
2351ATP synthase subunit B S. solfataricus AAK40880
2458.5HSP60 subunit S. shibatae AAG37273.1
3048Unknown
3116NDUnknown
3214.4NDPutative transcription regulator S. solfataricus AAK40413
3313.8NDTranscription factor nusA S. solfataricus AAK40563

Spots refer to those numbered in Figure 2. Proteins in Figure 2 which are not included in this table were not subjected to sequencing due to their low intensities in the gels.
Spot up-regulated in cells exposed to Cu and Cd.
ND: not determined.
Infinity symbol indicates that proteins were expressed only in presence of copper or cadmium.
M. thermoautotrophicus: Methanobacterium thermoautotrophicus; M. acetivorans: Methanosarcina acetivorans; S. shibatae: Sulfolobus shibatae; P. torridus: Picrophilus torridus; P. aerophilum: Pyrobaculum aerophilum; S. acidocaldarius: Sulfolobus acidocaldarius.