Differential Susceptibility to Propofol and Ketamine in Primary Cultures of Young and Senesced Astrocytes

Figure 3

Cell count dose response of young astrocytes to DMSO, propofol, and ketamine. The average number of young astrocytes per dose after seven hours of incubation in escalating strengths of different media (30 μM, low; 300 μM, high; 100 μM of DMSO and propofol + DMSO not shown), expressed as percent of best growth in control media for each set of experiments and presented as means, 95% confidence intervals. Control (N = 4; white bar), low DMSO corresponding to 74% DMSO used to prepare 30 μM propofol (N = 3; light gray bar, left), high DMSO corresponding to 74% DMSO used to prepare 300 μM propofol (N = 6; light gray bar, right), low propofol + DMSO (N = 3; 30 μM dark gray bar, left), high propofol + DMSO (N = 6; 300 μM dark gray bar, right), low ketamine (N = 3; 30 μM closed bar, left), and high ketamine (N = 3; 300 μM closed bar, right). ^∗∗p < 0.01 vs control; ⁺p < 0.05 vs DMSO; ^#p < 0.05 vs propofol + DMSO. ANOVA with Bonferroni correction for post hoc comparisons.