Expression and Function of the Chemokine, CXCL13, and Its Receptor, CXCR5, in Aids-Associated Non-Hodgkin's Lymphoma
Figure 2
Representative expression of CXCR5 and CXCL13 protein in AIDS-NHL. Tissue arrays containing sections from numerous AIDS-NHLs were examined for expression of CXCR5 (a) or CXCL13 (b) by immunohistochemistry, as noted in Section 2. For CXCR5, an HRP/Fast Red system was used for color development (red); for CXCL13, Vectastain Elite ABC reagent and DAB were used (brown). Arrays were counterstained with hematoxylin. Sections representative of typical CXCR5 and CXCL13 staining patterns in AIDS-associated Burkitt lymphoma (AIDS-BL, indicated as “Burkitt’s”) and AIDS-associated diffuse large B cell lymphoma (AIDS-DLBCL, indicated as “LCL”) are shown. Both AIDS-BL and AIDS-DLBCL show strong expression (3+) of CXCR5; AIDS-DLBCL shows strong expression (3+) of CXCL13, whereas AIDS-BL shows more moderate expression (2+). The sections shown representing CXCR5 and CXCL13 expression in AIDS-DLBCL came from the same tumor, an AIDS-DLBCL in the maxillary sinus. Normal sinus tissue (*) is unstained. For each tumor section stained for CXCR5 or CXCL13 expression, a negative control using an isotype-specific, non-cross-reactive, antibody is shown on the right. All sections are shown at x100 original magnification, except that the Burkitt lymphoma in panel B is shown at x200 original magnification. Pictures were taken using an Olympus DP11 camera attached to an Olympus BX51 bright field microscope, and recorded on a Smart Media digital card. The 10x and 20x objective lenses (UPlan Apo, Japan) had apertures of 0.40 and 0.70, respectively. Pictures were edited for publication using Adobe Photoshop 6.0 and Canvas 5.0.3 (ACD Systems of America, Inc.).
