Analytical Performances of Human Immunodeficiency Virus Type 1 RNA-Based Amplix® Real-Time PCR Platform for HIV-1 RNA Quantification

Table 6

Run duration (extraction plus amplification and lecture), number of samples per run, test cost and equipment cost for HIV-1 RNA-based Amplix real-time PCR assay, and the reference COBAS AmpliPrep/COBAS TaqMan HIV-1 test v2.0.

Platform	Run duration (hour)	Number of samples per run	Cost/test (US $)	Equipment cost (US $
COBAS AmpliPrep/COBAS TaqMan (Roche Molecular Diagnostics)	6	176 per 8 hour-day continuous loading	16	COBAS TaqMan 48: 45,000–100,000; COBAS AmpliPrep: 80,000–150,000
HIV-1 RNA-based Amplix real-time PCR (Biosynex)	3	48 per 6 hour-day continuous loading	15	ExiPrep™ 16 Dx: #18,000–25,000; Thermocycler NG 48: #12,000–20,000

to UNITAID 2015 [6], the cost per test in the Global Access Program for HIV viral load testing is around 10 US $.