Combination of Interleukin-27 and MicroRNA for Enhancing Expression of Anti-Inflammatory and Proosteogenic Genes

Figure 4

Osteoblastic cells. MC3T3E1(14) cells cultured in the presence of ascorbic acid and beta-glycerol phosphate were cotransfected with miR control or experimental (miRlet7f, miR-10a, miR-20b, miR-21, miR-210, miR-17, and miR-29b) and luciferase reporter vectors for detecting IL17a, TNF, and TRAF2 expression or SMAD2/3, STAT3, and STAT1 activity. (a) Effect of IL-27 (50 ng/ml) on luciferase expression. (b) Effect of experimental miRNA on luciferase expression. (c) Effect of miRNA+IL27 combination on luciferase expression. Black rectangle frames (□) highlight the data with significant expression changes () relative to negative control (miR ctrl); osteoblastic cells, differentiating MC3T3E1-14 cells treated with differentiation supplements for 7 days. Yellow arrowheads, proposed best combinations of miR and IL-27. Color bar, range of fold change in expression, from green (−4.0-fold change or decrease) to red (2.8-fold change or increase).