Figure 2: Conversion of glyoxal to glyoxylate in liver tissue homogenates. Incubations contained 0.2 mM glyoxal, 50 mM TRIS pH 9.0, 100 mM KCl, 1 mM EDTA, 1 mg human liver lysate, 10 minutes, 37°C. Reaction additions: 1 mM NAD+, 1 mM NADP+, 1 mM disulfiram. Glyoxylate was measured in perchloric acid extracts by reversed phase HPLC after derivatization with 10 mM phenylhydrazine.