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Advances in Urology
Volume 2013, Article ID 836121, 6 pages
Research Article

Weight Loss and Melatonin Reduce Obesity-Induced Oxidative Damage in Rat Testis

1Gaziosmanpasa University, Faculty of Medicine, Department of Urology, 60100 Tokat, Turkey
2Sakarya University, Faculty of Medicine, Department of Biostatistics and Medical informatics, 54100 Sakarya, Turkey
3Gaziosmanpasa University, Faculty of Medicine, Department of Biochemistry, 60100 Tokat, Turkey
4Gaziosmanpasa University, Faculty of Medicine, Department of Pathology, 60100 Tokat, Turkey

Received 26 June 2013; Accepted 7 August 2013

Academic Editor: James A. Brown

Copyright © 2013 Dogan Atilgan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aim. We aimed to evaluate the antioxidant effects of weight loss and melatonin on the obesity-induced oxidative damage in rat testes. Materials and Methods. 28 male Wistar albino rats were randomly divided into 4 groups, each consisting of 7 rats: control group (Group 1), obesity group (Group 2), obesity + MLT group (Group 3), and weight loss group (Group 4). Rats were weighed at the beginning and at the end of the study. Bilateral orchiectomy was performed and 5 cc blood samples were obtained from all of the rats. Superoxide dismutase (SOD), malondialdehyde (MDA), and protein carbonyl (PC) levels were analysed in the testicular tissues and serum. Spermatogenesis was evaluated with the Johnsen scoring system. Results. The testicular tissue and serum levels of MDA, PC, and SOD activity were increased in the obesity group in comparison to the sham operated group ( ). Weight loss and melatonin treatment ameliorated MDA, PC, and SOD levels in testicular tissue and serum significantly ( ). There was no significant difference between groups in terms of mean Johnsen score ( ). Conclusion. Experimentally created obesity caused oxidative stress and both melatonin and weight loss reduced oxidative stress parameters in rat testes.