Acridine Orange and Flow Cytometry: Which Is Better to Measure the Effect of Varicocele on Sperm DNA Integrity?

Figure 1

Fluorescence microscopy of acridine orange destained cells shows that spermatozoa displaying green fluorescence were considered to be with normal DNA content, whereas sperms displaying a spectrum of yellow-orange to red fluorescence were considered to be with damaged DNA. Original magnification ×200.