Acridine Orange and Flow Cytometry: Which Is Better to Measure the Effect of Varicocele on Sperm DNA Integrity?

Figure 2

Flow cytometric detection of sperm DNA chromatin condensation. (a) Dot plot histogram (FL3A versus FL3W) obtained after propidium iodide staining of spermatozoa. Cells gated in R1 region were analyzed, while debris and aggregates were excluded from the analysis. The fluorescence intensity of cells (spermatozoa) was measured in R1 gate. (b) Histogram of fluorescence intensity of spermatozoa from normal control person. (c) Histogram of fluorescence intensity of spermatozoa from infertile person with varicocele.

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