Acridine Orange and Flow Cytometry: Which Is Better to Measure the Effect of Varicocele on Sperm DNA Integrity?

Table 1

Clinical and laboratory data of studied groups (mean ± SD).

Variables	Varicocele group ()	Fertile group ()	value
Sperm count (mil/mL)	26.2 ± 2.7	74.2 ± 19.2	0.005
Motility A + B (%)	27.8 ± 16.5	62.5 ± 11.9	0.02
Normal forms (%)	59.8 ± 13.6	71.03 ± 8.2	0.02
Sperm DFI (%) by acridine orange	32.4 ± 7.4	18.2 ± 4.8	0.003
GMFI of sperm DNA chromatin condensation by flow cytometry (%)	25.4 ± 8.8	12.8 ± 2.2	0.005
Mean age (years)	31 ± 8.2	30.2 ± 2	NS

Significant .
GMFI: the geometric mean fluorescence intensity.
DFI: DNA fragmentation index.