Acridine Orange and Flow Cytometry: Which Is Better to Measure the Effect of Varicocele on Sperm DNA Integrity?

Table 2

Pre- and postvaricocelectomy semen parameters, sperm DFI, and GMFI of sperm DNA chromatin condensation by flow cytometry.

Variables	Before varicocelectomy	After varicocelectomy	value
Sperm count (mil/mL)	26.2 ± 2.7	51 ± 4.2	0.05
Motility A + B (%)	27.8 ± 16.5	32 ± 7	NS
Normal forms (%)	59.8 ± 13.6	65.03 ± 8.0	NS
Sperm DFI (%) by acridine orange	32.4 ± 7.4	20 ± 4.1	0.05
GMFI of sperm DNA chromatin condensation by flow cytometry (%)	25.4 ± 8.8	22 ± 4.1	NS

Significant .