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Advances in Urology
Volume 2017, Article ID 3254980, 5 pages
https://doi.org/10.1155/2017/3254980
Research Article

Study of Testicular Structure in Fetuses with Prune Belly Syndrome

Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil

Correspondence should be addressed to Luciano A. Favorito; rb.moc.oohay@otirovaful

Received 15 October 2016; Accepted 3 May 2017; Published 21 May 2017

Academic Editor: Kostis Gyftopoulos

Copyright © 2017 Luciano A. Favorito et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. To compare the structure of the testis in fetuses with prune belly syndrome (PBS) to normal controls. Materials and Methods. We studied 6 testes obtained from 3 fetuses with PBS and 14 testes from 7 male fetuses. The testicular specimens were cut into 5-μm thick sections and stained with hematoxylin and eosin (HE), to observe the seminiferous tubules; Weigert’s solution to observe elastic fibers; and picrosirius red to observe collagen. The images were captured with an Olympus BX51 microscope and Olympus DP70 camera. The stereological analysis was done with the Image Pro and Image J programs. Means were statistically compared using the Mann–Whitney test (). Results. Quantitative analysis documented no differences () in number of seminiferous tubules (ST) in PBS testes (mean = 8.87%, ), when compared to the control (mean = 11.4%, ) and no differences () in diameter of ST in PBS testes (mean = 52.85 μm, ) when compared to the control group (mean = 53.17 μm, ), but we did observe a lower number () of Leydig cells in the PBS testes (mean = 67.03% and ) when compared to the control group (mean = 90.1% and ). Conclusions. Our study showed a lower concentration of Leydig cells in the triad syndrome fetuses.