Novel Roles of the Picornaviral 3D Polymerase in Viral Pathogenesis
Figure 5
Viral load in mice infected with EMCV or PRV. (a) Viral transcripts in EMCV infected mice. Nontransgenic and 3D transgenic FVB mice were infected with 40 plaque forming units of EMCV (VR-129B ATCC) intraperitoneally. Brain and spinal cord were harvested after 3 days and total RNA was isolated using Trizol reagent (Invitrogen). Viral transcripts for EMCV were quantified by real-time PCR using primers specific for the VP2 region of EMCV. Data were normalized to GAPDH transcripts and are expressed as mean relative viral transcripts + SEM ( for nontransgenic and 4 for 3D transgenic). Data was statistically significant using a two-tailed student’s -test assuming unequal variance. (b) Viral DNA in pseudorabies virus (PRV) infected mice. Nontransgenic and 3D transgenic FVB mice were infected with plaque forming units of PRV 152 intramuscularly in the left hind limb. Brain and spinal cord were harvested after 7 days and DNA was isolated. Viral DNA for PRV was quantified by real-time PCR using primers specific for the inserted EGFP in PRV 152. Data are expressed as mean relative viral DNA ( for nontransgenic and 3 for 3D transgenic) and were normalized to genomic IL-2 DNA.
