In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome Virus
Figure 1
Real-time RT-PCR validation of SAGE results. Validation by real-time RT-PCR on RNA from the in vitro PRRSV-infected PAMs samples used in the SAGE analysis of selected transcripts showing differential transcript abundance. Real-time RT-PCR transcript abundance results (filled squares; left -axis) expressed as the mean fold increase ± SEM in gene expression () relative to mock-infected cells and SAGE tag counts normalized to total tags per library (open diamonds; right -axis) are shown for transcripts. The “24 mock” result shows the real-time RT-PCR fold change in transcript abundance as a result of being in cell culture.
