Artificial 64-Residue HIV-1 Enhancer-Binding Peptide Is a Potent Inhibitor of Viral Replication in HIV-1-Infected Cells

Figure 6

Fluorescence microscopy of CEM-GFP cells. (a) unstimulated cells; (b) CEM-GFP cells stimulated with 0.32 μM PMA for 16 h at 37°C; (c, d, e) CEM-GFP cells stimulated with 0.32 μM PMA in the presence of 4 μM, 8 μM, and 12 μM R42, respectively, for 6 h at 37°C; (f, g, h) treatment of CEM-GFP cells with 0.32 μM PMA in the presence of 2.6 μM, 5.25 μM and 7.9 μM R64, respectively, for 6 h at 37°C drastically reduced the number of cells expressing the GFP gene; the arrows point to representative cells in which the GFP gene was silenced.