Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood Samples
Verifying XMRV rescue by culturing on DERSE cells with 22Rv1 supernatants and with XMRV-spiked human plasma. XMRV culturing under the following conditions: (i) 22Rv1 supernatant alone (black bars), (ii) 22Rv1 supernatant treated with CaCl2 + heparin (white bars), (iii) 22Rv1 supernatant spiked into human plasma treated with CaCl2 + heparin (gray bars). GFP-positive cells were analyzed by FACS at day 4 (a) and day 8 (b).
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