Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood Samples
Figure 3
Testing plasma samples from prostate cancer patients for replication competent XMRV. Twelve samples were blinded as to their X-SCA and ELISA results and were tested for replicating virus using the DERSE.L-iG-P assay in two separate experiments. Six samples were tested in experiment 1 at passages 10, 13, 17, and 22 (a). All passages were negative for XMRV while virus was recovered from the positive control samples (107 copies of XMRV from 22Rv1 cells spiked into human plasma). Six additional samples were tested in experiment 2 at passages 6, 9, and 16 (b). All passages were negative for XMRV while virus was recovered from the positive control samples.
