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Advances in Virology
Volume 2011, Article ID 343408, 11 pages
Research Article

Requirements for Human Respiratory Syncytial Virus Glycoproteins in Assembly and Egress from Infected Cells

Department of Pathology, University of Virginia, MR5 Building, P.O. Box 800904, Charlottesville, VA 22908-0904, USA

Received 27 March 2011; Accepted 16 May 2011

Academic Editor: Anthony P. Schmitt

Copyright © 2011 Melissa Batonick and Gail W. Wertz. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

A comparison of infectivity rates of HRSV with and without the F glycoprotein in different cell types. The ΔF virus utilizes the baculovirus GP64 protein for viral entry. HEp2, A549, and Vbac cells were infected with the ΔSH and ΔF viruses, both of which contain GFP. Infected cells, which were identified by GFP expression, were counted from 0 to 30 hours post-infection. All cell types tested were infected with the ΔSH virus. HEp2 cells were only minimally infected by the ΔF virus, while A549 cells and Vbac cells, allowed for a robust infection with the ΔF virus.

  1. Supplementary Figure