Advances in Virology / 2011 / Article / Tab 6

Research Article

Internally Controlled, Generic Real-Time PCR for Quantification and Multiplex Real-Time PCR with Serotype-Specific Probes for Serotyping of Dengue Virus Infections

Table 6

Summary of patient characteristics and sensitivity and specificity of PCR assays.

Patient characteristics and test parametersVietnamese patients with DENVIll-returned travelers with DENVOther causes of fever in travelersPatients positive for
Primary infectionSecondary infectionPrimary infectionSecondary infectionOFIHCV/HAVTotal

Patients (n)56721169(18)d136
Age; median (range)10.9 (6.6–12.8)18.9 (5.6–53.1)38.7 (17.4–62.6)33.2 (24.6–73.6)39.5 (26.3–64.0)unknown26.3 (6.6–73.6)
Sex (M/F)3/248/199/126/106/3unknown72/46
Days of illness at presentation; median (range)1 (1-1)1 (1–4)6 (1–12)8.5 (4–22)2 (1–22)
Days of DENV viremia; median (range)1 (1-1)1 (1–4)4 (1–11)7 (4–9)1 (1–11)
Patients ( ) with a positive result in
 Type-specific PCRa5661690ND96
 Generic PCRb3661580092
 Multiplex PCRc3631130ND80
Assay characteristics of Generic PCR
 Sensitivity; % (95% CI)60.0 (23.1–88.2)100.0 (94.5–100.0)93.8 (71.7–99.0)88.9 (56.5–98.0)95.8 (89.8–98.4)
κ-value0.7361.0000.9440.9240.846
 Specificity; %100.0100.0100.0

aThe results were earlier generated by the type-specific primer PCR according to according Laue et al. [11, 16, 17].
bInternally controlled generic real-time PCR for DENV.
cMultiplex PCR with type-specific probes was only done on samples with a positive generic PCRbresult.
d16 patients were antibody and PCR positive for HCV and 2 patients were antibody and PCR positive for HAV.
OFI: other febrile illness; HCV: hepatitis C virus; HAV: hepatitis A virus; M: male; F: female; 95% CI: 95% confidence interval, ND: not done.

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