Imaging Early Steps of Sindbis Virus Infection by Total Internal Reflection Fluorescence Microscopy
Single-particle tracking of a SINV virus binding to cell surface. Pseudocolor images showing that SINV attachment involved a two-step binding process. (a) A SINV-C′-YFP (arrow) was tracked under TIRF mode for 130 seconds. Its movement rate was measured manually frame-by-frame. Boxed images indicate no measurable movement by the virus. (b) Tracking of a SINV labeled with Qdot 605. (c) Four types of representative trajectories of Qdot-labeled SINV movement in BHK plasma membranes. a: immobile; b: mobile but confined in small areas (<0.5 μm diameter); c: mobile through long distances (>1 μm); d: mobile through medium distances (0.5–1 μm). Scale bar = 10 μm.
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