Advances in Virology

Research Article

No Evidence of XMRV or MuLV Sequences in Prostate Cancer, Diffuse Large B-Cell Lymphoma, or the UK Blood Donor Population

Table 1

Details of PCRs used to test blood sample.


Sample tested	PCR	Target	Primers and probes	Cycles ()	Reagents

540 DNAs from whole blood	XMRV Taq Man	gag	XMRV Probe, F, R	60	Qiagen QuantiTect Probe kit
540 DNAs from whole blood	SBCMV Taq Man	SBCMV plasmid	SBCWMVCPF, SBCWMVCPR, SBWMV237F	45	ABgene ABsolute QPCR ROX mastermix
540 DNAs from whole blood	PDH Taq Man	PDH human gene	PDH Probe, F, R	45	ABgene ABsolute QPCR ROX mastermix
600 NAs from whole blood 400 NAs from plasma minipools	XMRV/pMuLV RT Taq Man with BMV RT Taq Man	gag BMV	P2, F3, R4 BMV Probe, F, R	45	Qiagen QuantiTect Probe RT-PCR kit

The TaqMan assay conditions were 15 min at 95°C (15 secs 95°C, 1 min 60°C) × cycles. 400 nM concentrations of primers, and 200 nM probes were used in all the TaqMan assays with the exception of the CDC MuLV Taq Man where the concentration of each probe was 100 nM and the PDH TaqMan where the primer concentrations were 50 nM.