In Silico and In Vitro Comparison of HIV-1 Subtypes B and CRF02_AG Integrases Susceptibility to Integrase Strand Transfer Inhibitors

Table 1

Amino acid variations at the positions putatively affecting the susceptibility to INSTI in 4 isolated HIV-1 subtype CRF02_AG IN coding sequences.

Position	B consensus	Subtype CRF02_AG
		N1 (33CR)	N2 (49CR)	N4 (52CR Q148K)	N3 (68CR)
14	K	R	K	K	R
112	T	V	V	R	V
125	T	A	A	A	A
134	G	N	N	G	G
136	K	T	T	K	T
206	T	S	T	S	S
283	S	G	G	S	S

Compared with HIV-1 subtype B IN, seven variations present at positions 14, 112, 125, 134, 136, 206, and 283 of CRF02_AG 33CR IN; five variations at positions 112, 125, 134, 136, and 283 of CRF02_AG 49CR IN; five variations at positions 14, 112, 125, 136, and 206 of CRF02_AG 68CR IN; CRF02_AG 52CR Q148K has two variations at positions 125 and 206, and an INSTI-resistant mutation Q148K, the R112 was not considered.