The Symptom and Genetic Diversity of Cassava Brown Streak Viruses Infecting Cassava in East Africa

Figure 3

RT-PCR amplification of CBSUV and CBSV genomes using newly designed primers (CBSVF2 in combination with CBSVR7 and CBSVR8). 1 = CBSUV-[UG:Kab4-3:07], 2 = CBSUV-[TZ:Kib10-2:03], 3 = CBSUV-[KE:Mwa16-2:08], 4 = CBSV-[TZ:Zan6-2:08], 5 = CBSV-[MZ:Nam1-1:07], 6 = CBSV-[TZ:Nal3-1:07], H = RNA extraction from a CBSD-free plant, − = negative water control, and + = a known CBSV RNA control from previous sample preparations. The size ladder at each border of the gel is the 100 bp molecular weight markers (New England Biolabs, UK).