Recombinant Varicella-Zoster Virus Vaccines as Platforms for Expression of Foreign Antigens
Figure 2
VZV-BAC approach to generate rVZV. BAC sequences are inserted into the VZV genome within the intergenic region between ORFs 11 and 12 and maintained within E. coli. A foreign gene of interest (goi) with promoter is inserted between ORFs 65 and 66. rVZV-BAC DNA is transfected into MeWo cells to generate infectious rVZV. BAC sequences flanked by lox P sites (*) may be removed from the VZV genome by Cre recombination.