Recombinant Varicella-Zoster Virus Vaccines as Platforms for Expression of Foreign Antigens

<table>VZV-BAC approach to generate rVZV. BAC sequences are inserted into the VZV genome within the intergenic region between ORFs 11 and 12 and maintained within <i>E. coli</i>.  A foreign gene of interest (goi) with promoter is inserted between ORFs 65 and 66. rVZV-BAC DNA is transfected into MeWo cells to generate infectious rVZV.  BAC sequences flanked by <i>lox P </i>sites (*) may be removed from the VZV genome by <i>Cre</i> recombination.     </table>

Advances in Virology

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Figure 2

Figure 2: Recombinant Varicella-Zoster Virus Vaccines as Platforms for Expression of Foreign Antigens 