Advances in Virology / 2014 / Article / Fig 1

Research Article

Mutations in the H, F, or M Proteins Can Facilitate Resistance of Measles Virus to Neutralizing Human Anti-MV Sera

Figure 1

Flow cytometry analysis of escape mutant Halle-SLAM 1.2 (H: D332G, T380I; F: no change; M: Y114H, I319T). Vero-SLAM cells infected with the escape mutant virus or transfected with phCMV plasmids expressing the mutated H protein, either alone or with a phCMV plasmid expressing the nonmutated F protein, in the presence of antifusion tripeptide FIP were analysed 1 day after infection (or after transfection) using the indicated antibodies. Each overlay histogram plot represents one representative experiment; the MFI histogram data represent the mean percentages standard deviations for three such experiments. These values were obtained by using nonmutated Halle virus or expressed nonmutated Halle proteins as controls. (a) and (b) escape mutant Halle-SLAM 1.2-infected cells; ((c) and (d)) and ((e) and (f)), cells transfected with phCMV.H.D332G/T380I and phCMV.H.D332G, respectively; (g) and (h) cells transfected with different phCMV.H mutants or infected with the escape mutant; ((i) and (j)) and ((k), (l)), cells cotransfected with phCMV.F + phCMV.H.D332G/T380I or phCMV.H.D332G, respectively.

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