Advances in Virology / 2014 / Article / Fig 4

Research Article

Mutations in the H, F, or M Proteins Can Facilitate Resistance of Measles Virus to Neutralizing Human Anti-MV Sera

Figure 4

Flow cytometry analysis of escape mutant Halle-SLAM 2.3 (H: A158V; F: no change; M: M239I). Vero-SLAM cells infected with the escape mutant virus or transfected with phCMV expressing the mutated H, in the presence of antifusion tripeptide FIP when necessary, were analysed 1 day after infection or after transfection using the four antibodies indicated. Each overlay histogram represents one experiment; the MFI histogram data represent the mean percentages standard deviations for three experiments. These values were obtained by using nonmutated Halle virus or expressed nonmutated Halle-H protein as controls. (a) and (b)) Escape mutant Halle-SLAM 2.3-infected cells; ((c) and (d)) cells transfected with phCMV.H.A158V.

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