Advances in Virology / 2014 / Article / Fig 6

Research Article

Mutations in the H, F, or M Proteins Can Facilitate Resistance of Measles Virus to Neutralizing Human Anti-MV Sera

Figure 6

Flow cytometry analysis of escape mutant Halle-CD46 3.3 (H: L136P; F: no change; M: K243E, S312P). Vero-SLAM cells infected with the escape mutant virus or transfected with various phCMV plasmids in the presence, when necessary, of the antifusion tri-peptide FIP, were analysed 1 day after infection (or after transfection) using the indicated antibodies. Each overlay histogram represents one experiment; the MFI histogram data represent the mean percentages standard deviations for three experiments. These values were obtained by using nonmutated Halle virus or expressed nonmutated Halle proteins as controls. ((a) and (b)) Escape mutant Halle-CD46 3.3-infected cells; ((c) and (d)) cells transfected with phCMV.H.L136P; ((e) and (f)) cells cotransfected with phCMV.H.L136P + phCMV.F; ((g) and (h)) and ((i) and (j)) cells cotransfected with phCMV.H.L136P + phCMV.F + phCMV.M.K243E/S312P or phCMV.M, respectively.

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