Advances in Virology / 2014 / Article / Fig 6

Research Article

Mutations in the H, F, or M Proteins Can Facilitate Resistance of Measles Virus to Neutralizing Human Anti-MV Sera

Figure 6

Flow cytometry analysis of escape mutant Halle-CD46 3.3 (H: L136P; F: no change; M: K243E, S312P). Vero-SLAM cells infected with the escape mutant virus or transfected with various phCMV plasmids in the presence, when necessary, of the antifusion tri-peptide FIP, were analysed 1 day after infection (or after transfection) using the indicated antibodies. Each overlay histogram represents one experiment; the MFI histogram data represent the mean percentages standard deviations for three experiments. These values were obtained by using nonmutated Halle virus or expressed nonmutated Halle proteins as controls. ((a) and (b)) Escape mutant Halle-CD46 3.3-infected cells; ((c) and (d)) cells transfected with phCMV.H.L136P; ((e) and (f)) cells cotransfected with phCMV.H.L136P + phCMV.F; ((g) and (h)) and ((i) and (j)) cells cotransfected with phCMV.H.L136P + phCMV.F + phCMV.M.K243E/S312P or phCMV.M, respectively.
205617.fig.006

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19.