Structural Differences Observed in Arboviruses of the Alphavirus and Flavivirus Genera
Figure 12
Temperature and time dependence of Sindbis virus genome delivery into BHK cells. (a) Sindbis virus-cell complexes incubated and fixed at 15 min postinfection at several temperatures were analyzed by electron microscopy. The graph shows that, as temperature rises from 4°C to 37°C, there is a progressive increase in the population of empty particles at the cell surface (◆), representing the total population at 37°C, and a decrease in the population of full particles (▲), reaching zero at 37°C. (b) Interaction of Sindbis virus with BHK cells at 4°C for 15 to 60 min. The percentage of empty particles increases from 30% at 15 min to 66.6% at 60 min (◆), and the population of full particles decreases from 70% to 33.4% over the same time period (▲). Data are shown as the means of triplicate samples of two independent experiments. Error bars are standard errors of the means (SEM). From Vancini et al. 2013 [133].
