Structural Differences Observed in Arboviruses of the Alphavirus and Flavivirus Genera

Figure 15

Cryo-EM maps reconstructed from class I to IV particles present in the DENV2 sample incubated at 37°C. The surfaces of the maps (above) and their central cross-sections (below) are colored according to their radii (shown in the lower panel). The white triangle represents an icosahedral asymmetric unit, and the corresponding 2-, 3-, and 5-fold symmetry vertices are indicated. Maps generated from the class I particles are similar to the previous published DENV-2 maps. Class II and III maps showed that the particles in these classes have bigger radii, indicating that the virus had expanded. There are protruding densities on the virus surface between the 5- and 3-fold vertices (white arrows). The class IV map showed very poor density on the E protein layer, indicating that the E protein had lost its icosahedral symmetry. With permission from Fibriansah et al. [175].