Research Article
The Antiviral Effect of High-Molecular Weight Poly-Gamma-Glutamate against Newcastle Disease Virus on Murine Macrophage Cells
Table 2
Primer sets used to quantify antiviral gene mRNA expression.
| | Genes | Primers | | Forward | Reverse |
| | GAPDH | 5′-TGACCACAGTCCATGCCATC-3′ | 5′-GACGGACACATTGGGGGTAG-3′ | | IFN-β | 5′-TCCAAGAAAGGACGAACATTCG-3′ | 5′-TGCGGACATCTCCCACGTCAA-3′ | | Mx1 | 5′-GATCCGACTTCACTTCCAGATGG-3′ | 5′-CATCTCAGTGGTAGTCAACCC-3′ | | IRF-3 | 5′-GTGCCTCTCCTGACACCAAT-3′ | 5′-CCAAGATCAGGCCATCAAAT-3′ | | IRF-7 | 5′-AAGCTGGAGCCATGGGTATG-3′ | 5′-GACCCAGGTCCATGAGGAAG-3′ | | ISG15 | 5′-CAATGGCCTGGGACCTAAA-3′ | 5′-CTTCTTCAGTTCTGACACCGTCAT-3′ | | GBP1 | 5′-AAAAACTTCGGGGACAGCTT-3′ | 5′-CTGAGTCACCTCATAAGCCAAA-3′ |
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