Figure 5: IgG isotypes; (a) IgG1, (b) IgG2b, and (c) IgG2a antibody response against RSV specific antigens. Serum samples (PBS, RSV infected, and PF DNA-immunized mice) were collected from BALB/c mice on days 0, 14, 28, and 49 and IgG isotypes were detected by ELISA. Data is presented as an average of triplicates performed twice; error bars represent standard deviations. Significantly different () from PBS group; significantly different () from PBS and PF DNA groups. values () were calculated using ANOVA, Tukey’s test.