Phage-Displayed Peptides Selected to Bind Envelope Glycoprotein Show Antiviral Activity against Dengue Virus Serotype 2

Figure 5

Biological activities of active peptides in Vero cells. (a) Cytotoxicity of active peptides. Cells were incubated with increasing concentrations of the peptide for 1 h and washed away and cells incubated in maintenance medium for 24. Then cytotoxicity was estimated from ATP levels using luminescence. The viability was estimated by comparing with nontreated cells. Data is presented as means and standard deviations of two independent experiments. Medians were compared between the nontreated control and all other groups using Kruskal-Wallis with Dunn’s multiple comparison test. (b) DENV2 infectivity inhibition by peptides, as shown in a plaque reduction assay. The reduction in the number of plaques was estimated by comparison with virus-infected cells alone. The peptide SPSTHWK is presented as negative control since it showed no consistent inhibitory activity against the virus. Data is presented as means and standard deviations of two independent experiments.

(a)

(b)