The Study of Viral RNA Diversity in Bird Samples Using De Novo Designed Multiplex Genus-Specific Primer Panels

Table 3

Results of the bird samples sequencing with prior enrichment using the primers panel. The number of viral reads and total number of reads per corresponding sample are shown. For three samples (B11, B23, and B24, highlighted in bold) there was a specific primer pair in the panel present for the amplification of the detected genus. Closest viral homologs names and their GenBank IDs are shown.

Bird ID	Viruses (closest homologs) detected	Host; specie	GenBank IDs of the detected viruses	Number of viral reads in the sample	Total number of reads obtained for the sample
B11	Duck-dominant coronavirus∣Avian coronavirus∣Bird droppings coronavirus	Taiga Bean Goose	AKQ98474.1, AKQ98475.1, APU51837.1, AIY51827.1, CAH69463.1	4,342	108,833
B23	Duck-dominant coronavirus∣Avian coronavirus∣Bird droppings coronavirus	Taiga Bean Goose	AKQ98474.1, AKQ98475.1, APU51837.1, AIY51827.1, CAH69463.1	83,730	194,156
B24	Duck-dominant coronavirus	Taiga Bean Goose	AKQ98474.1, AKQ98475.1	4,554	90,430
B27	Sanxia water strider virus 16	Temminck’s Stint	YP_009337377.1	4,062	103,488
B27	Fowl aviadenovirus C∣Turkey aviadenovirus B	Temminck’s Stint	ACL68145.1, ANB27700.1, ALY06332.1, ALY06333.1	54	103,488
B27	Cimodo virus	Temminck’s Stint	YP_009059075.1	160	103,488
B46	Watercress white vein virus ∣ Turnip yellow mosaic virus	Taiga Bean Goose	AFC95826.1, AMH40128.1	636	28,468
B49	Circovirus	Taiga Bean Goose	AEL87792.1	180	149,230
B58	Lake Sarah-associated circular virus-32	Taiga Bean Goose	ALE29729.1	10	42,348
B66	Duck aviadenovirus B	Fieldfare	YP_009047166.1	198	159,604
B68	Duck adenovirus A	Common tern	AGS11269.1, NP_044717.1	95,872	256,549
B69	Tunis virus	Taiga Bean Goose	AMT75434.1	16	126,648