Detection of HIV-1 and Human Proteins in Urinary Extracellular Vesicles from HIV+ Patients

Table 2

LC/MS/MS analysis of EV HIV proteins.

Accession	# AAs	MW [kDa]	Calc. pI	Description	Coverage	# peptides	Score A0	Coverage A0	# peptides A0
gi38491705	192	22.7	10.1	Vif protein [human immunodeficiency virus 1]	13.54	12	9.22	13.54	4
gi73913089	104	11.7	10.1	Gag protein [human immunodeficiency virus 1]	14.42	15	6.27	14.42	4
gi58374258	869	98.1	8.8	Envelope glycoprotein [human immunodeficiency virus 1]	1.5	5	5.63	1.5	3
gi183197180	404	45.8	8.4	Pol protein [human immunodeficiency virus 1]	3.47	3	5.27	3.47	3
gi255984636	160	18.1	5.3	Reverse transcriptase [human immunodeficiency virus 1]	7.5	4	4.80	7.5	2
gi256012108	114	13.5	5.7	Nef protein [human immunodeficiency virus 1]	14.04	3	4.53	14.04	2
gi9756252	524	60.4	8.7	Pol precursor [human immunodeficiency virus 1]	4.01	2	4.46	4.01	2
gi67082579	191	22.3	9.4	Reverse transcriptase [human immunodeficiency virus 1]	10.47	2	4.43	10.47	2
gi2290009	852	96.7	8.5	Envelope glycoprotein [human immunodeficiency virus 1]	7.16	11	4.33	5.87	3
gi167886806	25	2.7	8.7	Rev protein [human immunodeficiency virus 1]	56	4	4.29	56	2
gi23344577	99	10.6	9.4	Protease [human immunodeficiency virus 1]	12.12	5	4.36	12.12	2
gi4324808	1437	161.9	8.3	Gag-pol polyprotein [human immunodeficiency virus 1]	2.51	7	4.05	1.6	2
gi222533599	73	8.0	9.1	Env C2V3 protein [human immunodeficiency virus 1]	23.29	4	3.85	23.29	2
gi71060450	206	23.7	6.3	Negative factor [human immunodeficiency virus 1]	4.85	4	3.84	4.85	2
gi37935985	85	10.3	4.8	Vpu protein [human immunodeficiency virus 1]	11.76	4	3.84	11.76	2
gi108860432	870	98.8	8.5	gp160 [human immunodeficiency virus 1]	3.33	2	3.74	3.33	2
gi114801226	209	24.4	10.1	Tat protein [human immunodeficiency virus 1]	5.26	2	3.70	5.26	2
gi22596451	341	38.6	8.0	Truncated envelope glycoprotein [human immunodeficiency virus 1]	3.81	4	3.68	3.81	2
gi183200570	342	38.7	9.2	Truncated pol protein [human immunodeficiency virus 1]	3.51	2	3.68	3.51	2
gi34786230	176	19.8	9.6	gp120 protein [human immunodeficiency virus 1]	10.8	3	3.60	10.8	3
gi1002239	104	11.5	8.9	Envelope glycoprotein, v3 region [human immunodeficiency virus 1]	18.27	2	3.54	18.27	2
gi77168129	95	11.3	7.6	Vpr protein [human immunodeficiency virus 1]	13.68	3	3.40	13.68	2
gi222532593	129	14.7	8.4	Gag p17 protein [human immunodeficiency virus 1]	8.53	3	2.61	8.53	1
gi219688191	132	14.9	10.2	Matrix protein [human immunodeficiency virus 1]	9.09	6	2.56	9.09	1
gi255687141	288	32.1	7.7	Integrase [human immunodeficiency virus 1]	9.03	1	2.52	9.03	1
gi222532161	132	15.0	9.5	Gag p17 protein [human immunodeficiency virus 1]	9.09	6	2.45	9.09	1
gi37934078	573	65.0	9.0	Gag-pol fusion polyprotein [human immunodeficiency virus 1]	2.79	2	2.42	2.79	2
gi405003	207	23.1	7.7	gp120 [human immunodeficiency virus 1]	12.08	1	2.41	12.08	1
gi54792352	213	23.7	5.6	Gag polyprotein [human immunodeficiency virus 1]	9.39	2	2.34	9.39	2
gi3885826	132	14.9	9.6	p17 matrix [human immunodeficiency virus 1]	11.36	2	2.31	11.36	1

Accession # = NCBI NR database, #AAs = total number of amino acids in the protein entry, MW = molecular weight of the protein in kDa, Description: description from the NCBI database, and Peptides = total number of unique peptides found.