Characterization of Canine Adenovirus Type 2 Virus Infection Pattern in Canine and Human Cell Lines

Figure 4

GFP Expression analysis in Chinese Hamster Ovary CHO K1 cells and human cell lines PC3, SKOV3, HepG2, LS 174, and LS 180 at 100 MOI by flow cytometry. Cells were transduced by Adenovirus CAV2 and Ad5G/L at 100 multiplicity of infection (MOI 100: 100 virus particles per cell). GFP expression in cells was analyzed by flow cytometry 48 hours after Ad5G/L infection. The cell population was gated for live and single cells. The GFP + cells were determined in comparison to cell with no adenovirus infection. This figure is a representative of three independent infection experiments.